MicroSort Gender Selection Facts and Guidelines


     MicroSort Gender Selection

Facts and Guidelines

What is MicroSort®?
MicroSort® is the only scientifically proven preconception method that helps to increase the chances of having a boy or a girl. MicroSort® separates sperm into two groups: the sperm that produces a girl (X bearing) and sperm that produces a boy (Y bearing).

What is the MicroSort® technology based on?
MicroSort® technology is based on the fact that the X chromosome is substantially larger than the Y chromosome. Human sperm cells having an X chromosome will contain approximately 2.8% more total DNA than sperm cells having a Y chromosome.

How does MicroSort® separate the sperm?
The sperm cells are separated with the help of a flow cytometer, using a special staining. The sample is processed then stained with a fluorescent dye, which attaches temporarily to the DNA in each sperm cell. The stained cells are then sent through the flow cytometer, where they travel single file through a laser beam that makes them fluoresce (glow). The X bearing sperm cells that took in more stain will glow brighter than the Y bearing cells that took in less stain. The computer software is able to identify the brighter (X bearing) and dimmer (Y bearing) glowing cells and direct the flow cytometer to select the cells requested to be sorted.

How can the sorted sperm cells be used?
There are different ways the sorted sperm may be used:

1) Intrauterine Insemination (IUI): On the ovulation day the semen specimen is produced to be sorted. The intrauterine insemination with the sorted specimen takes place immediately after the sort.

2) in vitro Fertilization (IVF) with optional Intracytoplasmatic Sperm Injection (ICSI): The semen specimen can be frozen and ship to the lab, or preferably produce on the day of the sort. The sorted sample may be used the same day of the sort, or it may be frozen for future use.

3)  IVF with optional ICSI and Preimplantation Genetic Diagnosis (PGD): As above, the sample can be sorted fresh or previously frozen. The sorted sample can be use the same day of the sort or have it frozen for future use.

What is the minimum amount of cells needed for MicroSort®?
With IUI the minimum amount is 100 million Total Motile Cells (TMC), for IVF or ICSI the minimum amount is 70 million TMC. These values refer to the total amount of cells in the sample, regardless of the volume.
For IVF or ICSI the amount of cells can be achieved by freezing more than one ejaculate. If there are previous semen analysis, you can forward them to our lab and our staff will inform you if the amount of cells is above the limit or offer different options.

Can a sample be frozen and shipped to or from MicroSort® lab?
Yes, samples could be frozen and shipped to MicroSort® lab for sorting and/or shipped from the MicroSort® lab once they are sorted and frozen (Recommended just for IVF with optional ICSI cycles).

Freezing and thawing does reduce the number of viable sperm cells available for sorting, it is recommended to freeze multiple ejaculates to give a total of at least 200×106 TMC. In some cases, the patient may choose to travel to MicroSort® lab to produce a fresh specimen instead of freezing multiple ejaculates. Depending on the quality of the sample at least 100 x103 TMC are frozen in each vial, when
possible. If needed a Dry Shipper could be provided by the lab to transport samples.

Can a frozen sample be used for IUI?
Because of the reduced number of viable cells due to freezing and thawing, and the lower progression post thaw; only IVF and ICSI procedures are recommended for frozen sperm. Studies are under way to increase the viability of frozen sperm and hopefully IUI option will be offered in the future.

Can donor sperm be use for MicroSort® procedures?
Yes, donor sperm may be used for both IUI and IVF or ICSI in MicroSort®. Because of the high quality of donor sperm, and the high freezing and thawing proven capacity of the cells; the donor sperm may be able to be used for IUI.
For IVF or ICSI it is always possible to use a frozen donor sperm because of the low number of sperm the process requires.

How many sperm cells are sorted for each procedure?
In the case of IVF or ICSI with a fresh sample, only a few cells are required. When the procedure is done the same day of the sort, approximately 100 x103 cells are sorted. More cells are sorted (300-500 x103) if requested to freeze the remaining sample for further use. IVF or ICSI with frozen samples is used in the case the sperm is sorted in advance. When possible, multiple vials may be frozen from one sorted specimen. For this procedure depending on the quality of the specimen, at least 100 x103 TMC are frozen in each vial. For IUI, depending on the quality of the sample a total of 300 – 500 x103 sperm cells are sorted. On average the motility of the sorted samples is about 75 %, though this varies from individual to individual.

What is the success rate with MicroSort?


MicroSort IUI

MicroSort IVF+ICSI
















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